Anti HSP-10 staining on stem cells

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Quality of Results

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Cell Biology and Neuroscience
University of California, Riverside
graduate student

Company:

Santa Cruz Biotechnology

Product Name:

Anti HSP-10 antibody

Catalog Number:

sc-20958

Info: View Product View Product Specs

Browse Similar Products: HSP-10 Antibodies

The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response.  Specifically, I used this product to stain for heat shock protein -10 in stem cells fixed with paraformaldehyde. The antibody stained very well.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraformaldehyde fixed stem cells

Primary Incubation

1:50 in 5% serum, overnight at 4 degrees Celscius

Blocking Agent

5% serum

Secondary Incubation

1:200 for 1 hour at room temperature

Tertiary Incubation

NA

Detection

Nikon TI Eclipse microscope

Results Summary

Great product. Gave very clean staining.

Additional Notes

None

Related Categories

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Summary

The Good

Very clear staining. Antibody was provided in 1 ml vial, so it goes a long way.

The Bad

None

The Bottom Line

A great product with great technical support.

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