HSP-60 antibody staining on stem cells

Overall

Quality of Results

Ease-of-Optimization

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Cell Biology and Neuroscience
University of California, Riverside
Graduate student

Company:

Santa Cruz Biotechnology

Product Name:

Anti HSP-60 antibody

Catalog Number:

sc-1052

Browse Similar Products: HSP-60 Antibodies

The  antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response.  Specifically, this product was used to visualize heat shock protein -60 in paraformaldehyde fixed stem cells. The antibody worked very well.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraformaldehyde fixed stem cells

Primary Incubation

1:50 in 5% serum, overnight at 4 degrees Celsius

Blocking Agent

5% serum

Secondary Incubation

1:200 for 1 hour at room temperature

Tertiary Incubation

NA

Detection

Nikon TI Eclipse microscope

Results Summary

The florescence was very clean and specific. The technical support department was kind enough to provide a protocol for antigen retrieval which was initially thought to be required to make the antigen accessible.  However, I was able to get brilliant staining even without antigen retrieval.

Additional Notes

None

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Summary

The Good

Very specific and clean binding.

The Bad

None

The Bottom Line

Great product accompanied by great technical support.

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