DDB-2: Excellent Antibody

Overall

Quality of Results

Ease-of-Optimization

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Oral Biology
University of IL at Chicago
Graduate Student

Company:

Cell Signaling

Product Name:

DDB-2 Antibody

Catalog Number:

5416

My research project focuses on the role of NER-factor DDB2 as a regulator of epithelial to mesenchymal transition. DDB2 is a DNA Damage Binding protein. Its expression is downregulated in several cancers. We have been able to detect this protein very easily with this antibody by Western Blot in many mammalian cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Colon cancer HCT116 cell line

Primary Incubation

1:2000 O/N at 4 deg in 5% BSA

Blocking Agent

5% BSA

Secondary Incubation

1:3000 2hr at RT

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

Good specific bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works like a charm

The Bad

Not for IP

The Bottom Line

Great antibody at great price.

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