Excellent Antibody to Detect Human MMP-13

April 17, 2014

Overall

Quality of Results

Ease-of-Optimization

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Cardiology
BIDMC
Postdoc

Company:

Anaspec

Product Name:

Anti - MMP - 13 (Hinge)

Catalog Number:

54406

The goal of that study was to measure the protein amount of MMP-13 in human heart samples

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human heart

Primary Incubation

Dilution at 1/1000, incubation at 4 degrees overnight

Blocking Agent

Milk 5%

Secondary Incubation

HRP Anti-rabbit secondary antibody, 1/2000, 1 hour at room temperature

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody gives a single band at the right size. (around 60 kDa)

Additional Notes

None

Related Categories

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Summary

The Good

Specificity

The Bad

None

The Bottom Line

Excellent antibody! I would definitely recommend to use it for the detection of Pro-MMP-13 in human samples.

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