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Overall

Performance

Ease-of-Optimization

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Saint Louis University
Pharm & Phys Science
Post Doc

Company:

Life Technologies

Product Name:

QuantStudio7 Flex

Catalog Number:

4485701

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We typically perform 20-40 gene expression experiments each week. This machine is fantastic in that you can create templates, drop in your plate and go, analyze your data in a variety of ways and mix detectors for a variety of samples in one plate. We have always used applied biosystems instruments and recently upgraded to this model. We are starting to use it for a variety of assays including ligand binding melt shifts, FRET, and high resolution melting. It is extremely efficient, performs a variety of experiments, and the software is very user friendly.

Experimental Design and Results Summary

Application

QPCR

Starting Material

N/A

Protocol Overview

We use a standard SYBR green protocol

Tips

Make sure to keep up with calibrations every few months

Results Summary

The software will do almost all of the analysis for you or you can just export the data into excel and do the analysis yourself. Extremely user friendly!

Features Summary

Interchangeable block system, no lasers

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Make sure you calibrate properly

The Bottom Line

Excellent product. Very consistent, easy to use. The support techs are also very helpful during set up and training.

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