Strong Signal: Anti-BRM IF

April 15, 2014

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Biological Science
Florida State University
Assistant Professor

Company:

Santa Cruz Biotechnology Inc.

Product Name:

Anti-Brm (N-19)

Catalog Number:

sc-6450

The purpose of this experiment was to identify an antibody that was suitable for detection of BRM in human cells by indirect immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human hTERT-RPE1 cells

Primary Incubation

1:400 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20

Blocking Agent

Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20

Secondary Incubation

1:100 dilution of FITC Donkey anti-Goat secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature

Tertiary Incubation

No tertiary incubation was performed

Detection

Direct labeled Donkey anti-Goat antibodies

Results Summary

The antibody stains well and detects a nuclear pattern consistent with this chromatin protein.

Additional Notes

Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100

Image Gallery

Summary

The Good

The signal was very strong and as expected for the distribution of this chromatin protein

The Bad

No drawbacks were evident with this antibody

The Bottom Line

This antibody is good for immunofluorescence. Provision of 1.0ml ensures countless staining experiments can be performed.

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