Easy and Fast Estimation of Glucose Uptake

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Quality of Results

Ease-of-Optimization

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Whitehead Institute for Biomedical Research
biology
postdoctoral associate

Company:

Cayman Chemical

Product Name:

Glucose Uptake Cell-Based Assay Kit

Catalog Number:

600470

Cayman's Glucose Uptake Cell-based Kit allows simple, fast and accurate estimate of uptake of a fluorescent glucose analog by cells. Results can be acquired and analyzed by conventional microscopy flow cytometry, or spectrometry.

Experimental Design and Results Summary

Application

Microscopy, Flow cytometry

Starting Material

Cultured cells

Protocol Overview

Incubate cells with the fluorescent glucose analog, harvest cells, wash and fix. Analyze by microscopy or FACS

Tips

None

Results Summary

The FACS plot shows peaks corresponding to the amount of fluorescent analog taken up by the cells under two different conditions. Black curve represents negative control.

Additional Notes

None

Related Categories

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Summary

The Good

Easy, fast and accurate

The Bad

None

The Bottom Line

Easy, fast and accurate

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