Transit-293 Transfection Reagent

February 27, 2014

Overall

Quality of Results

Ease-of-Optimization

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Touro University-CA
Basic Sciences, College of Osteopathic Medicine
Professor

Company:

Mirus

Product Name:

Transit-293

Catalog Number:

MIR 2704

This experiment was a first-time test of the efficacy of Transit-293

Experimental Design and Results Summary

Application

Transfection

Starting Material

293T cells

Protocol Overview

293T cells were plated at a density of 300,000 cells/well in a 24 well plate and allowed to grow overnight in DMEM, supplemented with antibiotics and 10% FCS. I used 0.5ug GFP in pCS2 along with the reagent according to the kit protocol.

Tips

Plating 300,000 cells in a 24-well plate gave a completely confluent monolayer, this may have affected the transfection efficiency. I recommend plating 150,000-200,000 cells.

Results Summary

At 48 hrs post-transfection, cells were examined on a inverted fluorescent microscope for GFP expression. Despite the blurry photo (our 'scope is quite fussy) it is clear that GFP is well expressed in the cell monolayer. I estimate that the transfection efficiency was 50% and this was done without optimization. In a repeat experiment using 150,000 cells/well, the transfection efficiency was about 90%.

Additional Notes

None

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Summary

The Good

Easy to use protocol, short incubation time, can be used directly on cells in serum-containing medium. Company also provides free samples for testing!

The Bad

None. Toxicity was non-existent. Price is higher compared to similar lipofection reagens (>$200 for 0.4ml)

The Bottom Line

For a first-time, out-of-box test, Transit-293 performed impressively.

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