CD206 (MMR) Antibody: Works Out of the Box

Overall

Quality of Results

Ease-of-Optimization

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CSB
MGH
postdoc

Company:

BioLegend

Product Name:

Biotin anti-mouse CD206 (MMR) Antibody

Catalog Number:

141714

The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters progenitors counts. We selected Biotin anti-mouse CD206 (MMR) from BioLegend specifically because CD206 is expressed in myeloid cells and can be found in adult spleens. Using this product I was able to identify different subsets and focus on progenitor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen

Primary Incubation

1:50 30' on ice

Blocking Agent

Fcblock

Secondary Incubation

Streptavidin-PerCP, 1:250, 15' on ice

Tertiary Incubation

None

Detection

FC

Results Summary

No need to define the best working concentration. Not very bright and thus easy to compensate with PE and PE-Cy7.

Additional Notes

None

Image Gallery

Summary

The Good

Useful for multicolor panels

The Bad

Need higher concentrations of Ab

The Bottom Line

Good and flexible reagent

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