CD103 Antibody: Reliable

Overall

Quality of Results

Ease-of-Optimization

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CSB
MGH
postdoc

Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD103 Antibody

Catalog Number:

121422

Browse Similar Products: CD103 Flow Cytometry Antibodies

The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters progenitors counts. We selected Brilliant Violet 421™ anti-mouse CD103 from BioLegend specifically because CD103 is expressed in myeloid cells and can be found in adult spleens. Using this product I was able to exclude differentiated subsets and focus on progenitor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen

Primary Incubation

1:200 30' on ice

Blocking Agent

Fcblock

Secondary Incubation

None

Tertiary Incubation

None

Detection

FC

Results Summary

No need to define the best working concentration. Bright and easy to compensate with BV-510

Additional Notes

None

Related Categories

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Summary

The Good

Useful for multicolor panels

The Bad

None

The Bottom Line

Good reagent

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