CD150 (SLAM) Antibody: Works Out of the Box

Overall

Quality of Results

Ease-of-Optimization

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CSB
MGH
postdoc

Company:

BioLegend

Product Name:

Brilliant Violet 605 anti-Mouse CD150

Catalog Number:

115927

The aim of the research is to study bone marrow hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters progenitors counts. We selected Brilliant Violet 605™ anti-mouse CD150 from BioLegend specifically because CD150 is expressed in long-term hematopoietic stem cells (LT-HSC) and can be found in adult bone marrow. Using this product I was able to profile many different progenitor subsets at the same time.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

1:200 30' on ice

Blocking Agent

Fcblock

Secondary Incubation

None

Tertiary Incubation

None

Detection

FC

Results Summary

No need to define the best working concentration. Bright and easy to compensate with PE, BV-510 and BV-711

Additional Notes

None

Related Categories

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Summary

The Good

Easy

The Bad

So few ul in each tube

The Bottom Line

Go for it

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