Epithelial Phetotype Detection by E-Cadh

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Transduction Labs (BD Biosciences)

Product Name:

Ms anti E-Cadherin antibody

Catalog Number:

610182

E-Cadh is the most important marker of epithelial cells. Once SNA1 repressor triggers the Epithelial-mesenchymal transition (EMT), CDH1 gene is down regulated, and E-Cadh protein is lost. Epithelial cells undergo into important phonotypical changes and become mesenchymal cells.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

E7.5 mice embryos

Primary Incubation

1/200

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Anti Ms

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Good staining at membrane of epithelial cells.

Additional Notes

None

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Summary

The Good

Specificity.

The Bad

Any remarkable.

The Bottom Line

Antibody works perfectly.

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