Cell Cycle Analysis. CDK1 Detection by WB

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

BD

Product Name:

Anti CDK1

Catalog Number:

554161

We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle progression of SNA1 factor. We used this antibody for the analysis of that mechanism.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDCK cells

Primary Incubation

1/1000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Ms HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Unique intense band at predicted MW and few smears at higher MW

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

Good option for cell cycle analysis.

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