MPM-2 - Cell Cycle Analysis by IF

MPM-2 antigens are strongly expressed within mitotic cells. In eukaryotes, MPM-2 phosphoproteins have been found in the cytoplasm of mitotic cells as well as being localized to centrosomes, kinetochores and midbodies of the microtubule organizing centers. Also labelled are fibers of the mitotic spindle. MPM-2 has been shown to stain the core of condensed mitotic chromatin but not interphase chromatin. MPM-2 antigens have also been found in interphase cells. The intensity of immunofluorescent staining in interphase cells is relatively weak compared to mitotic cell staining and limited to discrete areas of the nucleus. A dramatic increase in MPM-2 reactivity occurs at the G2/M transition as shown by immuno-fluorescence microscopy and flow cytometry.