Qiagen AllPrep DNA/RNA Extraction Kit for FFPE

Protocol Overview

The sections are initially de-paraffinised by xylene and ethanol washes. Samples are then lysed and subjected to proteinase K digestion before centrifugation to separate the RNA-containing supernatant and DNA-containing pellet. At this stage, the material is separated and the RNA protocol is followed, while the DNA pellets can be processed subsequently or stored for extraction at a later date. The RNA extraction protocol involves incubating the supernatant at 80C, followed by coloum-based binding of RNA, DNase treatment, washing and the total RNA is finally eluted. MicroRNAs can also be extracted by addigina larger volume of ethanol during the procedure. The DNA extraction comprises lysing the pellet with proteinase K digestion and incubation at 90C, followed by column-based binding of genomic DNA, optional RNase-treatment, washing and eluting the DNA.