Loading Control for WB: Tubulin

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Cancer Research Program
Fundación IMIM
Lab manager

Company:

SIGMA

Product Name:

Monoclonal Anti-α-Tubulin antibody produced in mouse

Catalog Number:

T5168

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth family (zeta-tubulin) which is present only in kinetoplastid protozoa.[1] The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules.We use this antibody as loading control to normalize amount of protein in cell extracts by WB

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell extracts

Primary Incubation

1/15000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Ms HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Nice detection in all cell types tested.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

One of the best options to normalize loading amount.

Share your experience with other scientists. Write a Review! »

Join the discussion