Quantitative IL-10 ELISA

Overall

Quality of Results

Ease-of-Optimization

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University of Sri Jayawardenapura
Microbiology
PI

Company:

Mabtech

Product Name:

IL-10 ELISA kits

Catalog Number:

3430-1HP-10

We have used these ELISA kits in many experiments but basically to find out the serum IL-10 concentrations in patients with acute dengue infection (Malavige et al, PLOS Neg Dis 2013; Malavige et al, BMC infectious Dis, 2013). It is very easy to use and always gave comparable results. There was hardly any variation in the ODs of the standard curve between ELISA plates. We have probably done over 35 IL-10 ELISA plates by now.

Experimental Design and Results Summary

Application

Quantification of serum IL-10

Starting Material

Serum and plasma

Protocol Overview

We dilute serum of plasma 5 times before using it in this assay. This is because we found that IL-10 was present at very high concentrations in some patients (over the detection value) and also since we wanted to preserve patient samples.

Tips

This is a very user friendly kit

Results Summary

We found that serum IL-10 levels were significantly higher in patients with more severe forms of dengue. We have also used this kit to do serial samples in dengue patients (results not published).

Additional Notes

None

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Summary

The Good

It is easy to use and there is hardly any kit to kit variation

The Bad

None

The Bottom Line

Excellent product given at a good price

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