Cell Signalling
Akt antibody
#9272S
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Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473.Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor
Western Blot
MDCK cells
1/1000
Milk 5% in TBS-T
Anti Rb HRP
Not needed
ECL
Nice detection at 1/100.
None
Specificity.
Any remarkable.
This antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins.