GFP Detection by WB

January 20, 2014

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Abcam

Product Name:

Anti GFP

Catalog Number:

ab6556

Info: Supplier Page View ProductView Product Specs 

Browse Similar Products:  GFP Antibodies

GFP is a fluorescent protein that is used as protein tag in vitro.Its detection by WB can be used to normalize transfection or to detect proteins when do not exists any antibody to the fused protein

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDCK cell

Primary Incubation

1/5000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Rb HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Unique band in few seconds of exposure.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

High dilution. High specificity

The Bad

Any remarkable.

The Bottom Line

Any cross-detection with other proteins.

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