Cell Adhesion and E-Cadherin Antibody Review

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

BD

Product Name:

Anti E-Cadherin

Catalog Number:

610182

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. E.-Cadh or CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HT29 human cells

Primary Incubation

1/2000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Ms HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Single and strong band with 10ug of total extract of protein.

Additional Notes

None

Image Gallery

Summary

The Good

Specific signal. Strong signal

The Bad

Any remarkable.

The Bottom Line

A single band at 120 kDa is detected with few seconds of exposure.

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