The Good
This antibody produces a punctate state consistent with dendritic proteins. Quantification of the number of NR2A+ dendritic spines was consistent with the literature.
The Bottom Line
This antibody is excellent for localization of NMDA receptors in hippocampal dendritic spines. I tried several antibodies against the obligate NR1 subunit and none of those antibodies produced a reliable immunocytochemsitry.