P63 Antibody: Very Nice Results

Overall

Quality of Results

Ease-of-Optimization

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Urology
UW-Madison
Lab Manager

Company:

BioLengend

Product Name:

P63 (deltaN) Antibody

Catalog Number:

619002

We needed a basal cell marker to stain areas of epidermal differentiation in embryonic mouse urethras and tried a few different p63 antibodies before selecting this one as the best.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraffin-embedded embryonic mouse urethras

Primary Incubation

1:1000 dilution in blocking, O/N at 4C

Blocking Agent

2.5% Horse Serum

Secondary Incubation

1:200 Bethyl Goat anti-Rabbit HRP in blocking 30mins at RT

Tertiary Incubation

N/A

Detection

DAB

Results Summary

Great staining even after 1hr incubation

Additional Notes

None

Related Categories

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Summary

The Good

Cost effective and clean

The Bad

None

The Bottom Line

Really clean, nice results are easily attainable

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