Sigma
Mouse anti-GFP
G6539
Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through which severe stress disrupts learning and memory. CRH, in combination with neuronal activity and NMDA receptor activation, recruits the actin destabilizing protein, calpain, to break down the actin cytoskeleton resulting in spine loss. Identifying the downstream signaling from CRH receptors could reveal potential therapeutic targets for diseases such as post-traumatic stress disorder (PTSD), anxiety, and depression.
Immunofluorescence
Hippocampal pyramidal neurons
1:1,000
3% BSA + 0.1% Triton in PBS
1:400
None
IF
I used this antibody to amplify the GFP signal in hippocampal pyramidal neuron in culture that were infected with a lentivirus to induce the expression of GFP. This antibody allows for the visualization of dendritic spines and withstands confocal imaging well.
Dendritic spines are visible
Great antibody for amplification of GFP signal.