Insulin Detection in Pancreas

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

SIGMA

Product Name:

Monoclonal Anti-Insulin antibody produced in mouse

Catalog Number:

I2018

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and fat tissue to absorb glucose from the blood. In the liver and skeletal muscles, glucose is stored as glycogen, and in fat cells (adipocytes) it is stored as triglycerides.When control of insulin levels fails, diabetes mellitus can result.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mice pancreas

Primary Incubation

1/500

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Donkey Anti ms

Tertiary Incubation

Not needed

Detection

Alexa 555

Results Summary

Insulin protein is detected in pancreas' islet according to the literature.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

Nice antibody to use in IHF

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