Biolegend
Alexa Fluor® 647 anti-mouse/human CD44 Antibody
103017
Info: View Product View Product Specs
This antibody was used to sort for CD44-positive cells in primary human keratinocytes. I selected this antibody because it was conjugated and had been previously tested by the company.
Flow Cytometry
Primary human keratinocytes
0.25 ug per 10^6 cells in 100uL at 4*C for 30 minutes.
N/A
BD Aria; BD Diva software
The antibody worked well in staining my cells, with little or no background compared to the isotype control.
Very specific; low background; inexpensive
None.
This is a good, inexpensive antibody that is able to distinguish CD44 positive cells with minimal background.