CytoKeratin19 in Mouse Pancreas

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Quality of Results

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Abcam

Product Name:

Anti-Cytokeratin 19 antibody [EPNCIR127B]

Catalog Number:

ab133496

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. CK19 belongs to the intermediate filament family and serve as cell type marker in many tissues and in tumors, although several false positives should be considered.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice pancreas

Primary Incubation

1/200

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Leyca Powervision

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Strong cytoplasmic signal after few seconds of exposition. Any background.

Additional Notes

Retrieval: Tris buffer (pH 9)

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Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

Perfect antibody to work with.

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