Collagen Staining in Mouse

Overall

Quality of Results

Ease-of-Optimization

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Fundación IMIM
Cancer Research Program
Lab manager

Company:

Roche

Product Name:

Trichrome III Blue Staining Kit

Catalog Number:

05279364001

Collagen staining is very usual technique for in vitro diagnostic. Robust and reproducible method of staining is necessary for routinely use.

Experimental Design and Results Summary

Application

IHC on paraffin, PFA-fixed samples

Starting Material

Adult mouse gut

Protocol Overview

Kit is used in automated staining stations of same vendor (VENTANA - ROCHE)

Tips

Deparaffinate the sample previous to the use.

Results Summary

Kit is designed to be very friendly. Bar codes into the Kit components don't allow the user any confusion. Staining program is included in the automated stations, although limited variations in final color output can be reached.

Additional Notes

None

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Summary

The Good

Easy of use. Reproducible.

The Bad

Quite expensive.

The Bottom Line

The kits give a very quality staining method for connective tissue, muscle and collagen fibers.Once the samples are in the station, any other aid is required

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