a-SMA detection in mice (IHC-Paraffin)

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

SIGMA

Product Name:

Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse

Catalog Number:

A2547

Actin family of proteins play a role in cell motility, structure and integrity. Alpha actins being a major constituent of the contractile apparatus, mainly found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice embryo 18,5dpc (Skin)

Primary Incubation

1/800

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Leyca Powervision

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Specific and strong signal in skin is detected. Also (not shown) in many other tissues.

Additional Notes

Retrieval: citrate buffer (pH 6)

Related Categories

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Summary

The Good

Specificity. Strong signal.

The Bad

Any remarkable.

The Bottom Line

Clean detection at very diluted use of the antibody.

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