Axin2 Determination on Mouse Embryo (IHC-Paraffin)

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Abcam

Product Name:

Anti-Axin 2 antibody [EPR2005(2)]

Catalog Number:

ab109307

Axin2 protein, involved in Wnt signalling pathway, is important point in the down-regulation of Wnt signalling to the nucleus. Defects on its expression are related with colorectal cancer.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice embryo 18,5dpc

Primary Incubation

1/100

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

1/ 100 in blocking buffer

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Staining seems to be very specific ans consistent to the literature.

Additional Notes

Heat mediated retrieval needed (citrate pH6).

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Summary

The Good

Specificity.

The Bad

Any remarkable.

The Bottom Line

Good option in IHC detection.

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