Thermo Scientific Pierce Antibodies
CD82 Antibody
MA1-35963
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Human peripheral blood neutrophils were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton-X 100 + 1% BSA) overnight at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Immunofluorescence
Human peripheral blood neutrophils
1:100 for 12hr at 4C
1% BSA
Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) 1:1000 for 1hr at 25C
Not used
Confocal imaging
CD82 was co-localized with Lymphocyte function-associated antigen 1a (LFA 1a) in the cell membrane. Left - CD82, Middle - LFA 1a, Right - merge.
N/A
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Good signal with no background staining
None
This is a good antibody raised in mouse for the staining of CD82 in neutrophils by immunocytochemistry.