Life Technologies
H4K20me1 from Life Technologies
491023
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Histone H4 (mono methyl K20) modification in C. elegans embryo was detected in this study. To specifically detect cells with Histone H4 (mono methyl K20) modification in the embryo of worm, anti-H4K20me1 (491023) antibody from Life Technologies at 1/400 dilution was used.
Immunohistochemistry
Wholemount of C. elegans embryo
1:400 for 12hr at 4C
3% serum plus 0.3% Triton-X 100 in PBS
Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) 1:1000 for 1hr at 23C
Not used
Confocal imaging
H4K20me1 antibody counter-stained with nuclear marker DAPI. The nuclear part of the embryonic cells in C. elegans was labeled, which consistent with this modification in histone H4, acting in epigenetic regulation.
No antigen retrieval is required
Highly specific, no background staining
None
This is a very good antibody to detect H4K20me1 modification in C. elegans.