Aviva Systems Biology
CLK4 antibody - N-terminal region (ARP30645_P050)
ARP30645_P050
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Wetsern Blot with Clk4 antibody in HMEC-1
Western Blot
Lysates of HMEC-1
Overnight , 4°C (1:500 – 1:1000), in TBS-T; 1%BSA; 0,02 Na3
In 5 % low-fat milk solution in TBST
1h at room temperature with goat- anti rabbit – HRP conjugated (DAKO, 1:5000) in 5 % low-fat milk solution
None
ECL Plus Detection Reagens (Amersham), ECL – sol. 5 min incubation (blot)
Band at 57 kDa
Nothing
Price, quality
The product is adequate for WB detection