Eppendorf
Mastercycler ep gradient
5341
Our lab is investigating quantitative expression of various genes under different experimental conditions. We routinely employ qPCR for the analysis of different gene expression in both normal and cancerous cells before and after various drug treatments. We used Master cycler epgradient machine from Eppendorf for this purpose and obtained consistent result all the time.
Quantitative gene expression
cDNA synthesized from mRNA obtained from human cells.
Initially, mRNA was obtained from human cells and converted into cDNA. cDNA was loaded on to 96 well plate along with forward and reverse primers. Syber green (5 ul) was added and final volume was adjusted to 10 ul with mol bio grade water. Plate was centrifuged and loaded into the machine for analysis.
We used 2 ug of mRNA for the synthesis of cDNA and from this preparation, 2 ul of cDNA is used for the analysis.
We obtained high quality results for the expression of genes uder various conditions. The results were reproducible and thus we could able to publish these results in peer-reviewed journals.
Simple and user-friendly
None
Software is user friendly
Over all, the equipment is very help full in determining the expression of genes in different samples with very limited small amount of starting material.