Good Bcl-6 Antibody for Flow Cytometry

November 27, 2013

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Medicine
Weill Cornell Medical College
Research Associate

Company:

BioLegend

Product Name:

BCL6-PE antibody

Catalog Number:

648304

I was looking for a good BCL6 antibody for flow cytometry. After trying 5 different antibodies, I found this one to be very good and bright. Have used it for nearly a year.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

AML cell lines and primary cells

Primary Incubation

1:100, 1h at room temperature

Blocking Agent

FACS buffer (PBS+2%FBS) or FACS buffer with 2% BSA

Secondary Incubation

No secondary needed

Tertiary Incubation

Not needed

Detection

Flow cytometry

Results Summary

It shows good signal compared to negative control and decreased signal in siRNA transfected cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use, bright and strong signal

The Bad

Not for human specific, crossreacts with murine BCL6 as well

The Bottom Line

A good BCL6 antibody for flow. Recommend to all other users. Could be even better if more fluorochromes can be chosen.

Share your experience with other scientists. Write a Review! »

Join the discussion