Abcam
Anti-TBR2 / Eomes antibody - ChIP Grade
ab23345
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Embryonic neurogenesis was studied in the developing mouse brain. Tbr2 was used as a intermediate progenitor marker to measure the number of intermediate progenitor cells during development.
Immunohistochemistry
E16 mouse cortex tissue
1:400 for 24hr at 4C
3% BSA plus 0.1% Triton-X 100 in TBS
Alexa Fluor 546 Goat Anti-Rabbit IgG (H+L) 1:1000 for 1hr at 25C
N/A
Confocal imaging
Tbr2 antibody counter-stained with nuclear marker DAPI. The intermediate progenitor cells were located in the subventricular zone as literature suggested. The nuclear part of the cell was labeled, which consistent with its role as a transcriptional factor.
No antigen retrieval is required
Highly specific, no background staining
None
This is a very good antibody to detect intermediate progenitor cells in the mouse cortex.