IHC with Anti-beta Actin Antibody

November 12, 2013

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Abcam

Product Name:

Anti-beta Actin Antibody

Catalog Number:

ab52614

We use this antibody as WB loading control and we want to know if we can use it as routinarely IHC positive control during our normal workflow.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice embryo wt 20dpc in paraffin

Primary Incubation

Dilution 1/100. o/n, 4°C in TBS 1%BSA 1%FBS

Blocking Agent

TBS 1%BSA 1%FBS

Secondary Incubation

Leyca goat Powervision anti Rabbit. Undiluted. 25 min RT

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Perfect detection of the protein at 1/100 dilution. Any backgroud in tissues.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Intense signal. Not backgroud.

The Bad

Any important defect to refer.

The Bottom Line

We strongly believe that signal is specific.The antibody works fine.

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