Cell Signalling
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb
#4947
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The goal of this experiment was to compare antiviral signalling between two sets of MEFS (WT vs KO). MEFs were infected with Sendai virus and antiviral signalling was monitored by measuring the activity of phospho-IRF3. p-IRF3 is a well known marker for examining host antiviral response. A time course was performed with the Sendai virus and compared to uninfected (control)
Western Blot
Murine Embryonic Fibroblast cells (15ug) per well
1/1000, RT for 1hr in TBST 5% skim milk
TBST 5% skim milk
1/10000, RT for 1hr in TBST 5% skim milk
N/A
HRP- chemiluminescence
The antibody works great and is very specific. Compared to the control it was obvious that antiviral signalling was activated in the cells when infected with Sendai virus. Results confirm that antiviral signalling is comparable in both WT and KO MEFS.
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Good dilution and very specific
Haven't found one yet!
Great antibody and would definitely use this again. Can probably dilute Ab more and would still see a great result.