iCyt (Sony Biotechnology Inc.)
Brilliant Violet 421™ Anti-human CD3 Antibody
2102170
Multi-color flow cytometry can be tricky, especially when you're doing more than 6 colors. In order to free up some of the more commonly used channels, it's a good idea to use this antibody to stain CD3 hence utilizing a channel which isn't normally used on your flow instrument.
Flow Cytometry
Whole blood, or PBMC
No dilution needed. 5 µl per million cells or 5 µl per 100 µl of whole blood is sufficient
None needed
You'll need a flow cytometer that has a violet laser
Reasonable distinction is seen between CD3+ and CD3- cells. However, please remember to check the configuration on your cytometer before you buy this antibody.
BV421 is excited at 405nm. You'll need a bandpass filter 450/50 nm for detection
Frees up other channels
On the expensive side
Use it if your cytometer has the correct configuration.