PE anti-mouse Tim-1

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Surgery
UCLA
Assistant Researcher

Company:

Biolegend

Product Name:

PE anti-mouse Tim-1

Catalog Number:

119505

Our lab studies murine T cells; specifically we are interested in isolating the Tim-1+ subpopulation. Because there is no commercially available kit for for TIM-1+ T cell isolation, we combine the PE labeled TIM-1 mAb and a PE positive selection kit (Stemcell). Using this approach we are able to isolate a Tim-1+ subpopulation of cells that is about 90-95% pure.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Spleen T cells

Primary Incubation

30min at RT

Blocking Agent

1% Rat serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

99% specific

Additional Notes

None

Image Gallery

Summary

The Good

Equal to FACS data

The Bad

None

The Bottom Line

Very good product

Share your experience with other scientists. Write a Review! »

Join the discussion