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We were trying to identify different subsets of T cell memory in mice. The first step in the staining is to gate on CD3+ cells among the lymphocytic gate on mouse splenocytes. We did this using this antibody with no hassle! Optimization was straight-forward. 0.5uL of the antibody was enough to give us good separation of CD3+ and CD3- cells.
Mouse splenocytes
30 minutes on ice
Purified Anti-Mouse CD16/CD32 (15 minutes at room temp)
None
Flow cytometry
Using the lymhocytic gate, we can clearly distinguish CD3+ versus CD3- cells, as shown on the histogram on the right. On the left, an isotype control was used at the same antibody concentration (showing no staining).
Easy to optimize, good quality
A good, easy to use antibody!