eBioscience
Anti-Baculovirus Envelope gp-64
12-6991-80
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This antibody is used for the detection of the baculovirus envelope protein gp-64. GP-64 protein is upregulated on the surface of insect cells shortly after transfection with baculovirus. Insect cells are widely used for the production of recombinant mammalian proteins and this protein may be used as a marker of successful transfection without the need for full-scale protein preparation.
Detection via flow cytometry of gp-64 expressed at the cell surface
Hi5 insect cells, transfected with high-titer baculoviral supernatant
Seed one well (for each transfectant to be tested) with 2 x 10^6 Hi5 insect cells. Add 100ul of the baculoviral supernatant to be tested to each well and shake, covered and sealed for 24 hours at 27°C. Spin down and wash cells from each well twice. Resuspend in approx. 25ul of FACS buffer and add 0.5ul (0.1ug) anti-gp64 antibody to the cells and incubate at 4°C for 30min. Wash cells prior to flow cytometry analysis for staining.
None
N/A
PE-conjugated
This antibody can be used as a successful surrogate for recombinant protein production in an insect cell system. The pattern of staining allows the used to distinguish supernatant with high, medium and low baculoviral titre without the need for quantification of protein production.
A reliable and simple antibody that can save time and money in insect cell protein preps.
None.
A useful adjunct for labs producing recombinant proteins in insect cells.