EMD Millipore
Anti-TRA 1-60 antibody
MAB4360
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TRA-1-60 antigen is expressed upon the surface of human embryonic (ES) or human induced pluripotent stem (hiPS) cells. Specifically, TRA-1-60 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells. TRA-1-60 reacts with a neuraminidase sensitive epitope of a proteoglycan. Therefore, it has been used as a marker of human ES and hiPS cells. In addition, since TRA-1-60 is expressed on the surface of cells, it can be used in fluorescence activated cell sorting (FACS) analysis or immunofluorescence assays without fixing.
Immunocytochemical staining
Human ES and iPS cells
Anti-TRA 1-60 antibody diluted at 1:1000 in 3% bovine serum albumin (BSA) in phosphate buffered saline + 0.05% Tween-20 (PBST), incubated overnight in a cold room with rocking.
3% BSA in PBST, 3 hrs at room temperature with rocking.
Alexa-488 goat anti-mouse (Life Technologies cat #A21042), diluted 1:1000) in blocking buffer for 2 hrs at room temperature with rocking.
N/A
Olympus DSU spinning disk confocal microscope
Staining of the surface of hES/iPSC which did not overlapped with nucleus marker hoeschst. Staining was pretty strong with no background. Differentiated cells and original fibroblast cells did not stain with this marker.
None
Specific staining of human ES and iPS cells.
Good marker for hES/iPSC. This antibody gives us pretty good results as a pluripotent marker which correlates to mRNA level by RT-PCR as well.