R&D Systems
Human Erythropoietin Recepter-Phycoerythrin (EpoR-PE) Monoclonal Antibody
FAB307P
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We selected this antibody against the human erythropoietin receptor to study the change in receptor level during differentiation of erythrocytes.
Flow cytometry
UT-7/GM, a human cell line; not fixed. Cells were always kept in phosphate-buffered saline (PBS) + 0.5% bovine serum albumin (BSA) at room temperature, except for the incubation after antibody addition, when a 4°C temperature was used.
FAB307P; incubated for 45 min. We tested a series of dilutions and found that, with our cells, that using 1/3 of the recommended amount worked equally well.
PBS+0.5% BSA
N/A
Guava Easycyte and BD LSRII
Works quite well; very good concordance between qPCR and flow cytometry results. Strong signal that lasts.
Easy to use; little to no optimization required. Can use 1/3 of the recommended amount and still get it to work equally well.
No optimization required.
Price.
A great antibody for EpoR detection in UT-7/GM cells.