GPR120 Antibody from Novus Biologicals

April 19, 2013

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Immunology
Moffitt Cancer Center
Research Scientist
Western blot: The second band is the target band, at 42kD. The first and the third bands are non-specific bands.

Company:

Novus Biologicals

Product Name:

GPR120 Antibody

Catalog Number:

NBP1-00858

Image

Our goal in using this antibody was to look at the expression of mouse GPR120 in bone marrow. There are multiple mouse GPR120 antibodies available. We chose the Novus antibody because the company is known for their high quality of products.

Experimental Design and Results Summary

Applications

Western blotting

Sample

C57BL6 bone marrow

Primary Incubation

GPCR GPR120 antibody, 1:1000 dilution in 5% bovine serum albumin (BSA) in phosphate-buffered saline (PBST).

Blocking Agent

5% milk in PBST

Secondary Incubation

HRP-conjugated goat anti-rabbit antibody

Tertiary Incubation

N/A

Detection

Pierce ECL Western Blotting Substrate (from Pierce/Thermo Scientific, cat #32209), used according to manufacturer’s instructions.

Results Summary

Antibody detects the target band of GPR120 (42kD), although it has some non-specificity.

Additional Notes

None.

Related Categories

Image Gallery

Western blot: The second band is the target band, at 42kD. The first and the third bands are non-specific bands.

Summary

The Good

Can detect GPR120 in bone marrow samples.

The Bad

Specificity.

The Bottom Line

A good antibody for detection of GPCR120 in bone marrow.

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