Enzo Life Sciences
Fas (human) monoclonal antibody (APO-1-3)
ALX-805-020
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Our experimental goal was to determine relative levels of Fas protein production from a common laboratory T lymphocyte cell line (Jurkat). This antibody was successfully used to detect Fas by Western blot.
Western blotting
Jurkat whole cell lysates
Fas (APO-1-3) antibody supplied by Enzo Life Sciences (catalog #ALX-805-020). Antibody was diluted 1:1000 in blocking agent and incubated overnight at 4°C.
5% nonfat milk in Tris-buffered Saline with Tween (TBST), incubated 30 minutes at room temperature.
Goat anti-mouse IgG-HRP supplied by Sigma-Aldrich (catalog #A4416). Antibody was diluted 1:2000 in blocking agent and incubated for 1 hour at room temperature.
N/A
ECL Prime Western Blotting Detection Reagent (GE Amersham, #RPN2232) used following manufacturer instructions.
Fas protein is readily detectable with a molecular weight around 23 kDa. No background or non-specific bands are detected and Fas signal intensity is generally strong.
Jurkat cells were lysed with RIPA buffer.
Strong signal intensity.
None.
Antibody performs as expected.