GeneTex
Fas Ligand Antibody (N2C1)
GTX100644
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This antibody was used to test Fas Ligand (FasL) stability during various treatments as assessed by Western blot. We initially screened multiple antibodies from various vendors and this product worked the best.
Western blotting
Human recombinant FasL (aa 103-281) produced in HEK 293T cells.
Fas Ligand antibody (N2C1) supplied by GeneTex. Antibody was diluted 1:1000 in blocking agent and incubated overnight at 4°C.
5% nonfat milk in TBST, incubated 30 minutes at room temperature.
Goat anti-rabbit IgG-HRP supplied by Sigma-Aldrich(catalog #A6154). Antibody was diluted 1:2000 in blocking agent and incubated for 1 hour at room temperature.
N/A
ECL Prime Western Blotting Detection Reagent (GE Amersham, #RPN2232) used following manufacturer instructions.
No background or non-specific bands are detected and signal intensity is generally strong. Because this antibody is polyclonal, FasL fragments may also be detected.
0.2 ug protein can generally be detected on film using exposures of less than 1 minute. FasL is modified through the addition of 3 glycosylations, which result in full-length bands between 30-35 kDa.
Great signal with no non-specific bands.
None.
Works as expected.