Cell Signaling Technology
E-Cadherin (24E10) Rabbit mAb
3195
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The general aim of my research is focused on lysosomal storage disorders and Alzheimer’s disease. I work with animal models of the diseases, both as embryos and adults.
Western Blotting (WB) and Immunohistochemistry (IHC) of frozen tissue
Brain sections from mouse
E-Cadherin (24E10) Rabbit mAb, Incubate O/N @ 4°C ; 1:400 dilution in the blocking agent (see below) for IHC; 1:1000 for WB.
0.1% BSA, 0.5% Tween 20 in 1x PBS, 10% Normal Goat Serum for IHC; 5% dry milk works fine for WB.
Goat Anti-Rabbit (Jackson ImmunoResearch, cat#111-065-144), Incubate for 1 hour @ RT in blocking agent; 1:400 for IHC ; 1:10000 for WB.
N/A
For WB: Detection with SuperSignal* West Femto Chemiluminescent Substrate (ThermoScientific, cat#34096) on the ChemiDoc MP Imager with LEDs and Image Lab software (Bio-Rad, cat#170-8292) For IHC: Detection with Stable DAB (Invitrogen, cat#750118) under a regular light microscope; Watch for color change, can counterstain if you like.
For WB, you will see one band ~110kDa. There were no background bands. For IHC, staining is very obvious, minimal background as long as you don’t leave the Stable DAB on for too long.
Very easy to use antibody. You shouldn’t have any issues.
No background on WB. Strong signal, so dilutions of 1:1000 (WB) and 1:400 (IHC) are fine.
Does not work on paraffin sections.
A very good antibody for basic, exploratory experiements.