Abcam
Anti-Caspr Antibody
AB34151
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This antibody was used to reveal the structure of the Nodes of Ranvier of myelinated axons. The results showed that this antibody works excellently in both rat brain and cultured cells.
Immunocytochemistry for myelination cultures from embryonic rat brain
A dissociated neuron-oligodendrocyrte co-culture was prepared from embryonic day 16 rat forebrain and cultured for 40 days. Cells were fixed with 4% paraformaldehyde for 10 min and then rinsed with PBS.
Anti-Caspr, used at 1:300, diluted in PBS containing 10% goat serum, incubated overnight at 4°C.
10% normal goat serum; blocked at room temperature (RT) for 1 hour.
Biotin-labeled anti-rabbit IgG (from JacksonImmunoResearch, cat# 111-065-003), diluted at 1:250 in PBS containing 10% Goat serum. Incubated at RT for 1 hr.
N/A
Immunofluorescence. Using Avidin-Alex488 (Life Technologies, cat# S11223). Observed using a Olympus BX60 fluorescence microscope.
Using antibodies against Caspr and MBP for double-staining, the Nodes of Ranvier were revealed. This antibody produced strong positive signals with acceptable background.
Also works fine in frozen rat brain sections.
Excellent antibody, very consistent results, strong positive staining.
None.
Strongly suggested for studies of myelination.