The isolation and purification of RNA is challenging due to the delicate nature of RNA. Extracting total RNA, mRNA, or small RNA while avoiding contamination requires the careful selection of reagents and kits. Some of the numerous source-specific kits are available for isolation from blood, tissue, cultured cells, and plants. If isolating protein along with the RNA is desired, Ambion® PARIS™ kits from Life Technologies™ are available for comparison. Phenol is customarily used in RNA biology, but it is a hazardous reagent. Phenol-free. reliable and high-speed small RNA (ie, miRNA, siRNA) isolation kits are available. Some kits, like mirPremier® microRNA Isolation Kit from Sigma-Aldrich, are not specific to tissue or cell type. When comparing the range of RNA isolation and purification kits on hand, also take into account the number of preparations each kit offers.
Purpose was to extract total RNA from mammalian cells in culture (MEFS).
MicroRNAs (miRNA) were first identified in the 1990s and ever since miRNA ...
Thursday, August 22, 2013
Expression analysis has become commonplace. First, though, you need to harvest the RNA. Here are tips for ...
When isolating RNA, good lab technique is essential. RNA is more labile in comparison to fixed and sacrosanct ...
Dr. Stephen Bustin presents a MIQE-based evaluation of qPCR assay design and publications including qPCR including RNA integrity and purity, primer specificity, PCR and normalization of expression ... Watch Video
Thursday, December 10, 2009
EdgeBio introduces three new products in the PurElute™ DNA Purification line. Watch Video
Wednesday, April 13, 2011
Watch this webinar to learn how Agilent's SureSelect technology for RNA Target Enrichment can be applied to the field of transcriptome research. With an easy-to-use protocol, a single 24-hour ... Watch Video