Agilent's oligonucleotide aCGH platform offers genome-wide as well as customized profiling on 60-mer oligo CGH microarrays for human, mouse and rat. Agilent's CGH end-to-end solution consists of flexible microarray formats, optimized and easy-to-use protocol, high resolution microarray scanning and powerful analytics software.
The Sequenom MassARRAY® System offers MALDI-TOF mass spectrometry for high accuracy and sensitivity, robust chemistry for reproducible results, and advanced data analysis software to meet the needs of any genomic laboratory. This system utilizes a homogenous MassExtend (hME – single base extension) reaction termed iPLEX GOLD, for analysis of SNPs in multiplex format. Following the MassExtend reaction, oligonucleotides are printed onto specialized Spectro CHIPs and analyzed by the Sequenom mass spectrometer. The mass spectrometer provides a high level of sensitivity, capable of detecting allele frequencies as low as 3% on average.
The PCR reaction is performed with fluorescent dye-labeled primers, then the PCR fragments can be analyzed on a capillary DNA sequencing machine (ABI 3730xl DNA Analyzer), and the data is analyzed using AB GeneMapper software. By taking advantage of multiplexing by fragment size and dye color, and the ability of automated fluorescent genetic analyzer machines to autoload 16 samples at a time from 96-well or even 384-well microtitre plates, high throughput marker analysis experiments can be designed. This service has been applied to a wide variety of species and can accommodate any species for which microsatellite markers are available.